以人血清为对象,提取，纯化DNA？
答案:2 悬赏:70
解决时间 2021-03-06 00:24
- 提问者网友：独菊痴梦
- 2021-03-05 09:49
以人血清为对象,提取，纯化DNA？
最佳答案
- 二级知识专家网友：我的任性你不懂
- 2021-03-05 10:16
血清中DNA提取试剂盒
DNA Extractor (R) Kit
Wako Pure Chemicals
Grade: for Genetic Research
Regulation / Hazardous:
Storage condition: Keep at 2-10 degrees C.
Manufacturer: Wako Pure Chemicals
Summary
This is for research use only. Do not administer it to human.
Molecular Biology-DNA Extraction Kits.
Detects and quantitates contaminant DNA in serum and residual DNA in biopharmaceuticals. Employs a new extraction procedure for DNA purification from a single tube. This procedure, using Sodium Iodide (NaI) as a chaotropic agent, realizes DNA isolation of both high quality and high recovery from biological fluids without the use of phenol or chloroform. Complex and laborious manipulations are avoided when using this kit. In addition, this kit has a modified application for use with Molecular Devices' Threshold(TM) System.
PRINCIPLE of DNA EXTRACTION: A high concentration of chaotropic reagent, NaI, and an anionic detergent participate in solubilization of the proteins and lipids contained in biological samples.
After addition of isopropanol to the mixture, nucleic acids are co-precipitated with polysaccharide glycogen as a carrier, while other components remain soluble in the solution phase.
Ref.: Ishizawa, M., Kobayashi, T. and Matsuura, S., ''Simple procedure of DNA Isolation from Human Serum'', Nucleic Acids Res., 19, 5792 (1991).
UN 1170 and UN 1219
DNA Extractor (R) Kit
Wako Pure Chemicals
Grade: for Genetic Research
Regulation / Hazardous:
Storage condition: Keep at 2-10 degrees C.
Manufacturer: Wako Pure Chemicals
Summary
This is for research use only. Do not administer it to human.
Molecular Biology-DNA Extraction Kits.
Detects and quantitates contaminant DNA in serum and residual DNA in biopharmaceuticals. Employs a new extraction procedure for DNA purification from a single tube. This procedure, using Sodium Iodide (NaI) as a chaotropic agent, realizes DNA isolation of both high quality and high recovery from biological fluids without the use of phenol or chloroform. Complex and laborious manipulations are avoided when using this kit. In addition, this kit has a modified application for use with Molecular Devices' Threshold(TM) System.
PRINCIPLE of DNA EXTRACTION: A high concentration of chaotropic reagent, NaI, and an anionic detergent participate in solubilization of the proteins and lipids contained in biological samples.
After addition of isopropanol to the mixture, nucleic acids are co-precipitated with polysaccharide glycogen as a carrier, while other components remain soluble in the solution phase.
Ref.: Ishizawa, M., Kobayashi, T. and Matsuura, S., ''Simple procedure of DNA Isolation from Human Serum'', Nucleic Acids Res., 19, 5792 (1991).
UN 1170 and UN 1219
全部回答
- 1楼网友：白日梦制造商
- 2021-03-05 10:23
.dna提取的几种方法 (1).浓盐法 利用rnp和dnp在电解溶液中溶解度不同，将二者分离，常用的方法是用1m 氯 纳提取化钠抽提,得到的dnp粘液与含有少量辛醇的 氯仿一起摇荡,使乳化,再离心除去蛋白质,此时蛋白质凝胶停留在水相及氯仿相中间，而dna位于上层水相中,用2倍体积95%乙醇可将dna 钠盐沉淀出来. 也可用0.15 mnacl液反复洗涤细胞破碎液除去rnp,再以1mnacl提取脱氧核糖蛋白,再按氯仿---异醇法除去蛋白. 两种方法比较,后种方法使核酸降解可能少一些. 以稀盐酸溶液提取dna 时,加入适量去污剂,如sds可有助于蛋白质与dna 的分离。在提取过程中为抑制组织中的dnase对dna 的降解作用,在氯化钠溶液中加入柠檬酸钠作为金属离子的烙合剂.通常用.15mnacl,0.015m柠檬钠,并称ssc溶液,提取dna. （2）.阴离子去污剂法: 用sds或二甲苯酸钠等去污剂使蛋白质变性,可以直接从生物材料中提取dna .由于细胞中dna与蛋白质之间常借静电引力或配位键结合,因为阴离子去污剂能够破坏这种价键,所以常用阴离子去污剂提取dna. （3）.苯酚抽提法: 苯酚作为蛋白变性剂,同时抑制了dnase的降解作用.用苯酚处理匀浆液时,由于蛋白与dna 联结键已断,蛋白分子表面又含有很多极性基团与苯酚相似相溶。蛋白分子溶于酚相，而dna溶于水相。离心分层后取出水层，多次重复操作，再合并含dna 的水相，利用核酸不溶于醇的性质，用乙醇沉淀dna 。此时dna是十分粘稠的物质，可用玻璃漫漫绕成一团，取出。此法的特点是使提取的dna保持天然状态 . （ 4）.水抽提法: 利用核酸溶解于水的性质,将组织细胞破碎后,用低盐溶液除去rna，然后将沉淀溶于水中，使dna充分溶解于水中,离心后收集上清液.在上清中加入固体氯化钠调节至2.6m.加入2倍体积95%乙醇,立即用搅拌法搅出.然后分别用66% ٠80%和95%乙醇以及丙铜洗涤,最后在空气中干燥,既得dna样品.此法提取的dna中蛋白质含量较高,故一般不用.为除蛋白可将此法加以改良,在提取过程中加入sds.
我要举报
如以上问答内容为低俗、色情、不良、暴力、侵权、涉及违法等信息，可以点下面链接进行举报！
大家都在看
推荐资讯